浙江大学生命科学学院生物科学系导师：应盛华 正文

　　姓名：应盛华
　　性别：男
　　所在学院：生命科学学院
　　职称：副教授

　　工作研究领域：
　　主要从事真菌基因组学、真菌分子生物学及生防真菌研究与开发。
 
　　个人简介：
　　应盛华，男，1973年9月生，博士，浙江大学微生物研究所副教授、硕导。
　　1995年毕业于山东大学微生物系获学士学位，并分别于2000、2006年获浙江大学微生物学硕士和博士学位。
　　本人长期从事真菌抗逆、生防真菌侵染的功能基因以及遗传改造工作，目前正在积极开展真菌全基因组测序和功能基因组学的研究。
　　目前，本人已主持国家自然科学基金三项、“863”子课题一项、省部级重点项目一项和国家自然科学基金重点项目子课题一项，以及作为骨干成员参加“973”项目、国际合作项目各一项。
　　本人入选浙江大学青年骨干教师培养计划、荣获2006年度浙江大学校级奖教金、2007年度浙江大学优秀班主任称号、2007和2008年度浙江大学生科院先进工作者称号、2009年校百篇优秀毕业论文（本科毕设）指导教师奖。

　　主持项目：
　　1． 国家自然科学基金项目(30971960)球孢白僵菌耐热力与多菌灵抗性负关联的生理和分子机制 2010．01-2012．12 经费：32万
　　2． 国家自然科学基金项目(30600410)转苏云金芽孢杆菌营养期毒蛋白Vip3A的球孢白僵菌菌株及其侵染生物学2007．01-2009．12 经费：22 万
　　3． 浙江省重大科技专项（优先主题）(2008C02007-1)蔬菜和茶树重大害虫生防菌的创制和产业化研究 2008．07-2010．07 经费：100 万
　　4． 国家自然科学基金重点项目子课题 生防真菌适应季节性逆境的分子生态机制研究 2010．01-2013．12 经费：60万
　　5． “863”计划（子项目）(2007AA021305)核辐射环境微生物资源的开发利用 2007．06-2010．12 经费：37万
　　6． 浙江省教育厅基金 (G20020247)球孢白僵菌孢内活力相关多糖组分的研究 2002．7-2004．7 经费：1．5万
　　7． 浙江出入境检验检疫局 （浙大05-308104-4）产黄曲霉毒素真菌PCR检测技术的研究 2005．1-2007．12 经费：6．0万

　　主参项目：
　　1． “973”项目 害虫生防真菌致病、抗逆的分子机理与遗传改良 (2009CB118904) (2/8) 2009．01-2013．12 经费：450万
　　2． “863”项目 细菌、真菌类生物杀虫剂研究和创制 (2006AA10A212) (2/4) 2006．12-2010．10经费：54万
　　3． 科技部国际合作项目 (2007DFA31100) 中方第二负责人 2008．01-2010．12耐夏季高温和紫外辐射的高效杀虫剂研制 经费：160万

　　发表论文：
　　1． Gao Q, Jin K, Ying SH (equally first author), Zhang Y, Xiao G, et al． Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M． acridum． PLoS Genetics, 2011, 7: e1001264．
　　2． Qin Y, Ying SH (Co-corresponding), Chen Y, Shen ZC, and Feng MG． Integration of Insecticidal Protein Vip3Aa1 into Beauveria bassiana Enhances Fungal Virulence to Spodoptera litura Larvae by Cuticle and Per Os Infection． Applied and Environmental Microbiology, 2010, 76: 4611-4618．
　　3． Ying SH and Feng MG． A conidial protein (CP15) of Beauveria bassiana contributes to the conidial tolerance of the entomopathogenic fungus to thermal and oxidative stresses． Applied Microbiology and Biotechnology, 2011, 90:1711–1720．
　　4． Ying SH, Wang XH and Feng MG． Characterization of a thioredoxin (BbTrx) from the entomopathogenic fungus Beauveria bassiana and its expression in response to thermal stress． Canadian Journal of Microbiology, 2010, 56:1-9．
　　5． Ying SH and Feng MG． Means to mediating accumulation of hydrophobin-like proteins on the wall of Beauveria bassiana conidia for improved tolerance to thermal stress． Journal of General and Applied Microbiology, 2007, 53: 309-314．
　　6． Ying SH and Feng MG． Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana． Applied Microbiology and Biotechnology, 2006, 72: 206-210．
　　7． Ying SH． and Feng MG． Medium components and culture conditions affect the thermotolerance of aerial conidia of fungal biocontrol agent Beauveria bassiana． Letters in Applied Microbiology, 2006, 43: 331–335．
　　8． Ying SH and Feng MG． Relationship between thermotolerance and hydrophobin-like proteins in aerial conidia of Beauveria bassiana and Paecilomyces fumosoroseus as fungal biocontrol agents． Journal of Applied Microbiology, 2004, 97: 323-331．
　　9． Wang ZL, Ying SH, Feng MG． Gene cloning and catalysis features of a new mannitol-1-phosphate dehydrogenase (BbMPD) from Beauveria bassiana． Carbohydrate Research, 2010, 345: 50-54．
　　10． Xie XQ, Ying SH, Feng MG． Characterization of a new Cu/Zn-superoxide dismutase from Beauveria bassiana and two site-directed mutations crucial to its antioxidation activity without chaperon． Enzyme and Microbial Technology, 2010, 46: 217-222．
　　11． Li J, Ying SH, Shan LT, Feng MG． A new non-hydrophobic cell wall protein (CWP13) of Metarhizium anisopliae increases conidial hydrophobicity when expressed in Beauveria bassiana． Applied Microbiology and Biotechnology, 2010, 85: 975-984．
　　12． Yao SL, Ying SH, Feng MG, Hatting JL． In vitro and in vivo responses of fungal biocontrol agents to the gradient doses of UV-B and UV-A irradiations． BioControl 2010, 55: 413-422．
　　13． Liu Q, Ying SH, Feng MG and Jiang XH． Physiological implication of intracellular trehalose and mannitol changes in response of entomopathogenic fungus Beauveria bassiana to thermal stress． Antonie van Leeuwenhoek International Journal of General and Molecular Micriobiology, 2009, 95: 65-75． ．
　　14． Ye SD, Ying SH, Chen C and Feng MG． New solid-state fermentation chamber fits up bulk production of aerial conidia of fungal biocontrol agents on rice． Biotechnology Letters, 2006, 28: 799-804．
　　15． Zou G, Ying SH, Sheng ZC and Feng MG． Multi-sited mutations of beta-tubulin are involved in benzimidazole resistance and thermotolerance of fungal biocontrol agent Beauveria bassiana． Environmental Microbiology, 2006, 8: 2095-2106．
　　16． Jiang Q, Ying SH and Feng MG． Enhanced frequency of Beauveria bassiana blastospore transformation by restriction enzyme-mediated integration and electroporation． Journal of Microbiological Methods, 2007, 69: 512-517．
　　17． Shan LT, Wang ZL, Ying SH, Feng MG． Hydrophobicity-related protein contents and surface areas of aerial conidia are useful traits for formulation design of fungal biocontrol agents． Mycopathologia, 2010, 169: 483-494．
　　18． Xie XQ, Wang J, Ying SH, Feng MG． A new manganese superoxide dismutase identified from Beauveria bassiana enhances virulence and stress tolerance when overexpressed in the fungal pathogen． Applied Microbiology and Biotechnology, 2010, 86:1543–1553．
　　19． Feng MG, Chen C, Shang SW, Ying SH, Shen ZC and Chen XX． Aphid dispersal flight disseminates fungal pathogens and parasitoids as natural control agents of aphids． Ecological Entomology, 2007, 32: 97-104．
　　20． Zhang GZ, Feng MG, Chen C and Ying SH． Opportunism of Conidiobolus obscurus stems from depression of infection in situ to progeny colonies of host alatae as disseminators of the aphid-pathogenic fungus． Environmental Microbiology, 2007, 9: 859-868．
　　21． Feng MG, Pu XY, Ying SH and Wang YG． Field trials of an oil-based emulsifiable formulation of Beauveria bassiana conidia and low application rates of imidacloprid for control of false-eye leafhopper Empoasca vitis in southern China． Crop Protection, 2004, 23: 489-496．
　　22． Feng MG, Chen B and Ying SH． Trials of Beauveria bassiana, Paecilomyces fumosoroseus and imidacloprid for management of Trialeurodes vaporariorum (Homoptera: Aleyrodidae) on greenhouse grown lettuce． Biocontrol Science and Technology, 2004, 14: 531-544．

　　核心期刊
　　1． 应盛华和冯明光． 冠状耳霉产碱性蛋白酶及其活性． 菌物系统, 2000, 19(1): 128-131．
　　2． 应盛华和冯明光． 球孢白僵菌孢子乳悬剂的配方研制． 植物保护学报． 2001, 28(4): 345-351．
　　3． 应盛华． 球孢白僵菌制剂． 杭州科技, 1999, 6: 18-20．
　　4． 应盛华, 徐均焕和周杭松． 冠状耳霉碱性蛋白酶的生产及应用． 杭州科技, 2000, 4: 29-30．
　　5． 应盛华和冯明光． 真空干燥球孢白僵菌纯孢粉的活孢率、毒力与贮存期． 微生物学通报． 2002, 29(5): 42-47．
　　6． 应盛华和冯明光． 丝孢类虫生生防真菌制剂研制的技术瓶颈与改良途径． 中国虫生真菌研究与应用, 北京：中国农业科学技术出版社, 2003, 64-74．
　　7． 应盛华, 冯明光和许寿涛． 球孢白僵菌分生孢子乳悬剂对甘蓝上桃蚜的田间控制效果． 应用生态学报． 2003,14(4): 545-548．
　　8． 应盛华和冯明光, 许寿涛和马再波． 球孢白僵菌分生孢子乳悬剂对甘蓝上桃蚜的田间控制效果． 应用生态学报． 2003,14(4): 545-548
　　9． 应盛华和冯明光． 不同培养基质上球孢白僵菌孢子与菌丝的多糖含量变化． 菌物学报． 2005, 24(2):304-305．
　　10． 应盛华, 邓米霞和冯明光． 三种丝孢类生防真菌孢子类疏水蛋白的含量与组分比较． 浙江大学学报, 2006, 32(4): 401-405．
　　11． 僧明华, 应盛华和冯明光． 模拟昆虫中肠环境对球孢白僵菌分生孢子的影响． 浙江大学学报(农业与生命科学版), 2009, 35(3): 261-265．
　　12． 冯明光和应盛华． 不同含水量和温度下贮存中球孢白僵菌分生孢子活力与内源营养的衰变． 应用生态学报． 2002, 13(4): 439-443．
　　13． 杨旭芬, 应盛华和冯明光． 限制性内切酶介导的玫烟色拟青霉原生质转化体系构建． 浙江大学学报(农业与生命科学版), 2006, 32(6): 606-612．
　　14． 秦文彦, 程洁, 应盛华和冯明光． 可污染食品及饲料的产黄曲霉毒素真菌的多重PCR检测． 菌物学报, 2007, 26(3): 448-454．
　　15． 许寿涛, 应盛华和冯明光． 十种常用农药与球孢白僵菌的生物学相容性． 植物保护学报． 2002, 29(2): 158-162．
　　16． 许寿涛, 冯明光和应盛华． 球孢白僵菌对桃蚜接种后特定时间内的侵染率． 应用生态学报． 2002, 13(6): 701-704．
　　17． 张丽靖, 冯明光和应盛华． 球孢白僵菌液体培养条件下淀粉酶的产生及活性影响因子． 微生物学报, 2002, 42(6): 602-607．
　　18． 顿玉慧, 冯明光和应盛华． 新型球孢白僵菌孢子悬乳剂的高效杀蚜活性及其评价方法． 微生物学报, 2003, 43(6): 781-787．

　　会议论文：
　　1． Ying S-H, Feng M-G & Xu S-T． Field efficacy of emusifiable suspension of Beauveria bassiana conidia for control Myzus persicae population on Chinese cabbage． VIII International Colloquium on Invertebrate 　Pathology and Microbial Control． Foz do Igussu, Brazil, 18-23 August 2002, 64．
　　2． Feng M-G & Ying S-H． Interactive effect of water content and temperature on viability of Beauveria bassiana conidia and metabolism of internally reserved nutrients in storage． VIII International Colloquium on Invertebrate Pathology and Microbial Control． Foz do Igussu, Brazil, 18-23 August 2002, 112-113．
　　3． Xu S-T, Feng M-G & Ying S-H． Time-specific infectionrate of Beauveria bassiana conidia on Myzus persicae under controlled conditions． VIII International Colloquium on Invertebrate Pathology and Microbial Control． Foz do Igussu, Brazil, 18-23 August 2002,102-103．
　　4． Feng M-G & Ying S-H． Development of Beauveria bassiana-based mycoinsecticide for tea leafhopper control in China: Current status and prospects． The 38th annual meeting of Society for Invertebrate Pathology． University of Alaska, Anchorage, USA, Aug． 7-12, 2005, p80．
　　5． Ying S-H & Feng M-G Purification and gene cloning of a new hydrophobin-like protein that relates to thermal tolerance of aerial conidia of fungal biocontrol agents． The 38th annual meeting of Society for Invertebrate Pathology． University of Alaska, Anchorage, USA, Aug． 7-12, 2005, p50．
　　6． Ying S-H & Feng M-G Thiredoxin from the entomopathogenic fungi, Beauveria bassiana: Gene cloning, characterizationand functional expression． The 42nd annual meeting of Society for Invertebrate Pathology． Park city, Utah, USA, Aug． 16-20, 2009, p82．

　　专利成果：
　　1、应盛华、冯明光. 一种抗逆能力增强的生防真菌构建方法及抗逆能力增强的生防菌株. 公开(公告)号 CN101392259, 公开(公告)日 2009-03-25.
　　2、冯明光, 应盛华. 防治刺吸式口器害虫的菌株及其制剂. 中国发明专利号：ZL 200510060449.8 (2008-7-9)
　　3、 冯明光, 叶素丹, 应盛华, 陈春. 2005. 一种高纯度孢子粉生产工艺及所用的立式多层产孢箱. 中国发明专利号：ZL 200510050499.8 (授权日：2008-04-30)

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